【活动预告】染色质与表观遗传学系列讲座第5场-Rob Klose
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北京时间2021年2月2日（周二） 20:00-21:00
2021 Feb 2nd Tuesday 20:00-21:00 (Beijing Time)
参与方式 | Location
Zoom网络研讨会: 843 6931 3262
Bilibili直播：http://live.bilibili.com/22741871
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Webinar ID: 843 6931 3262
Bilibili Live: http://live.bilibili.com/22741871
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主讲人 | Speaker
Rob Klose
主讲人简介 | Speaker Biography
Rob Klose教授博士期间在爱丁堡大学师从Adrian Bird爵士，研究与甲基化DNA相结合的蛋白质。随后他来到张毅教授在北卡罗来纳大学教堂山分校的实验室进行了博士后培训，期间他鉴定了多种组蛋白脱甲基酶，并研究了它们在基因调控中的作用。Rob Klose教授现任牛津大学遗传学讲座教授兼系主任，他的实验室主要专注于研究CpG岛元件对基因表达的调控功能。
Dr. Rob Klose did his PhD with Professor Sir Adrian Bird at the University of Edinburgh where he studied proteins that bind to methylated DNA. He did his post-doctoral training with Professor Yi Zhang at UNC Chapel Hill where he identified histone demethylase enzymes and studied their roles in gene regulation. Rob is now the Professor and Chair of Genetics at the University of Oxford, where his group is focussed on understanding how CpG island elements function to regulate gene expression.
报告标题 | Title
Dissecting Polycomb-mediated gene repression
报告摘要 | Abstract
Polycomb Repressive Complex 1 (PRC1) and PRC2 function through chromatin-based mechanisms to repress gene expression and are essential for normal mammalian development. However, how PRC-dependent repression is achieved at the molecular level has remained poorly defined. Addressing this problem has been complicated by the interrelatedness of PRC1 and PRC2 activities at target gene promoters. Here, I will discuss our efforts using rapid perturbation coupled with time-resolved quantitative genomic approaches to examine the central determinants of Polycomb-mediated gene repression in mouse embryonic stem cells (ESCs).
主讲人发表论文摘选
Selected Publications
1) Nadezda A. Fursova, Anne H. Turberfield, Neil P. Blackledge, Emma L. Findlater, Anna Lastuvkova, Miles K. Huseyin, Paula Dobrinić, Robert J. Klose. BAP1 constrains pervasive H2AK119ub1 to control the transcriptional potential of the genome. bioRxiv 2020.11.13.381251; doi: https://doi.org/10.1101/2020.11.13.381251
2) Paula Dobrinić, Aleksander T. Szczurek, Robert J. Klose. PRC1 drives Polycomb-mediated gene repression by controlling transcription initiation and burst frequency. bioRxiv 2020.10.09.333294; doi: https://doi.org/10.1101/2020.10.09.333294
3) Hughes AL, Kelley JR, Klose RJ. Understanding the interplay between CpG island-associated gene promoters and H3K4 methylation. Biochim Biophys Acta Gene Regul Mech. 2020 Aug;1863(8):194567. doi: 10.1016/j.bbagrm.2020.194567. Epub 2020 Apr 29. PMID: 32360393; PMCID: PMC7294231.
4) Miles K. Huseyin, Robert J. Klose. Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy. bioRxiv 2020.04.25.061358; doi: https://doi.org/10.1101/2020.04.25.061358
5) Feldmann A, Dimitrova E, Kenney A, Lastuvkova A, Klose RJ. CDK-Mediator and FBXL19 prime developmental genes for activation by promoting atypical regulatory interactions. Nucleic Acids Res. 2020 Apr 6;48(6):2942-2955. doi: 10.1093/nar/gkaa064. PMID: 31996894; PMCID: PMC7102981.
6) Kriaucionis S, Klose RJ. ATACing DNA Methylation during Differentiation. Mol Cell. 2020 Mar 19;77(6):1159-1161. doi: 10.1016/j.molcel.2020.02.026. PMID: 32200796.
7) Blackledge NP, Fursova NA, Kelley JR, Huseyin MK, Feldmann A, Klose RJ. PRC1 Catalytic Activity Is Central to Polycomb System Function. Mol Cell. 2020 Feb 20;77(4):857-874.e9. doi: 10.1016/j.molcel.2019.12.001. Epub 2019 Dec 27. PMID: 31883950; PMCID: PMC7033600.
8) Rhodes JDP, Feldmann A, Hernández-Rodríguez B, Díaz N, Brown JM, Fursova NA, Blackledge NP, Prathapan P, Dobrinic P, Huseyin MK, Szczurek A, Kruse K, Nasmyth KA, Buckle VJ, Vaquerizas JM, Klose RJ. Cohesin Disrupts Polycomb-Dependent Chromosome Interactions in Embryonic Stem Cells. Cell Rep. 2020 Jan 21;30(3):820-835.e10. doi: 10.1016/j.celrep.2019.12.057. PMID: 31968256; PMCID: PMC6988126.
9) Turberfield AH, Kondo T, Nakayama M, Koseki Y, King HW, Koseki H, Klose RJ. (2019) KDM2 proteins constrain transcription from CpG island gene promoters independently of their histone demethylase activity. Nucleic Acids Res. 2019 Sep 26;47(17):9005-9023. doi: 10.1093/nar/gkz607. PMID:31363749
10) Fursova NA, Blackledge NP, Nakayama M, Ito S, Koseki Y, Farcas AM, King HW, Koseki H, Klose RJ. (2019) Synergy between Variant PRC1 Complexes Defines Polycomb-Mediated Gene Repression. Mol Cell. 2019 Jun 6;74(5):1020-1036.e8. doi: 10.1016/j.molcel.2019.03.024. PMID:31029541
11) King H.W., Fursova N.A., Blackledge N.P., Klose R.J. (2018) Polycomb repressive complex 1 shapes the nucleosome landscape but not accessibility at target genes. Genome Res. 28(10):1494-1507.
12) Dimitrova E., Kondo T., Feldmann A., Nakayama M., Koseki Y., Konietzny R., Kessler B.M., Koseki H., Klose R.J. (2018) FBXL19 recruits CDK-Mediator to CpG islands of developmental genes priming them for activation during lineage commitment. elife. 7,e37084
13) Long H.K., Rose N.R., Blackledge N.P., Klose R.J. (2018) Biochemical Identification of Nonmethylated DNA by BioCAP-Seq. Methods Mol Biol. 1766:15-29
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