推荐阅读文章 | 乳源耐甲氧西林金黄色葡萄球菌icaA/D基因缺失株的构建及其生物被膜形成能力与耐药性分析

图1  ica操纵子结构示意图

Figure 1  Schematic diagram of the ica operon

图2  同源重组质粒pMD19-T simple-icaAL-icaDR的PCR鉴定

Figure 2  PCR identification of homologous recombination plasmid pMD19-T simple-icaAL-icaDR

图3  基因敲除质粒pBT₂-icaAL-icaDR的鉴定

Figure 3  Identification of knockout plasmid pBT₂-icaAL- icaDR

图4  基因敲除质粒pBT₂-icaAL-icaDR的电转结果

Figure 4  The electroporation result of knockout plasmid pBT₂-icaAL-icaDR

图5  可疑菌落的抗性筛选结果

Figure 5  The suspicious colonies screened by resistance of antibiotics

图6  可疑菌落的PCR鉴定

Figure 6  PCR identification of the suspected colonies

图7  icaA/D基因疑似缺失株的组合PCR鉴定

Figure 7   Multiple-PCR identification of the suspected icaA/D gene deletion strain

图8  MRSA M5野生株的生物被膜形成过程

Figure 8  Biofilm formation process of the MRSA M5 wild strain

图9  MRSA M5-ΔicaA/D缺失株的生物被膜形成过程

Figure 9  Biofilm formation process of the MRSA M5-ΔicaA/D strain

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本期制作：赵彬涵