推荐阅读文章 | 金属离子对重组深渊滕黄单胞菌低温α-淀粉酶LamA的活性和稳定性影响

图1  不同金属离子对LamA热稳定性的影响

Figure 1  Effects of different metal ions on thermo-stability of LamA

图2  LamA与其他α-淀粉酶的蛋白序列比对

Figure 2  The protein sequence alignment of LamA and other α-amylases using the DNAMAN

图3  黄粉虫幼虫α-淀粉酶钙离子结合区域的蛋白结构模拟

Figure 3  Protein structure simulation of calcium ion binding region of Tenebrio molitor larval α-amylase

图4  可溶性淀粉平板检验LamA及突变蛋白酶活力

Figure 4  Enzyme activity assay of LamA and mutant proteins by soluble starch plate medium

图5  温度对LamA及突变蛋白活性的影响

Figure 5  Effects of temperature on the activity of LamA and mutant proteins

图6  不同温度对LamA及突变蛋白稳定性的影响

Figure 6  Effects of temperature on the stability of LamA and mutant proteins

图7  5 mmol/L钙离子条件下N120与LamA的热稳定性

Figure 7  Comparison of thermal stability of N120 and LamA under 5 mmol/L calcium ion condition