Green revertible leaf-color mutants are basical materials for studying the mechanism of chloroplast differentiation and development. We have obtained a green-revertible chlorina mutant named grc2 with every leaf greening independently, from an indica maintainer line T98B treated by ⁶⁰Co-γ radiation. Each leaf of grc2 is initially chlorotic, and then turns green after growing about 10 days. The mutant grc2 showed a new pattern of virescence which refreshed green regardless of its plant growth stage. Compared with the wild type T98B, the total chlorophyll and chlorophyll b content reduced significantly in the yellowish leaves of grc2 and chloroplast remained in the etioplast stage, suggesting that grc2 would probably be an essential gene functioning in the development of young leaves. Genetic analysis revealed that, grc2 was controlled by a single recessive nuclear gene. The gene of grc2 was fine mapped between STS markers S254 and S258 with a physical interval of 31 kb on the short arm of chromosome 6, by using 960 F₂ plants with mutant phenotype from a cross between grc2 and Nipponbare. This region contained five annotated genes that had not published. These results provides important information for studying in gene cloning and gene function of grc2.