Zhengke Wang;Sijie Chen;Jacky W.Y. Lam;Wei Qin;Ryan T.K. Kwok;Ni Xie;Qiaoling Hu;本忠 唐
Hong Kong University of Science and Technology;Zhejiang University;China Association for Science and Technology
发表时间:2013-6-5
期 刊:Journal of the American Chemical Society
语 言:English
U R L: http://www.scopus.com/inward/record.url?scp=84878625521&partnerID=8YFLogxK
There is a great demand for long-term cellular tracers because of their great importance in monitoring biological processes, pathological pathways, therapeutic effects, etc. Herein we report a new type of fluorescence "turn-on" probe for tracing live cells over a long period of time. We synthesized the fluorogenic probe by attaching a large number of tetraphenylethene (TPE) labels to a chitosan (CS) chain. The resultant TPE-CS bioconjugate shows a unique aggregation-induced emission (AIE) behavior. It is nonfluorescent when dissolved but becomes highly emissive when its molecules are aggregated. The AIE aggregates can be readily internalized by HeLa cells. The cellular staining by the TPE-CS aggregates is so indelible that it enables cell tracing for as long as 15 passages. The internalized AIE aggregates are kept inside the cellular compartments and do not contaminate other cell lines in the coculture systems, permitting the differentiation of specific cancerous cells from normal healthy cells.
工程与材料科学
化合物
医学与生命科学
Scopus度量
年份 | CiteScore | SJR | SNIP |
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1996 | |||
1997 | |||
1998 | |||
1999 | 3.438 | 2.106 | |
2000 | 3.589 | 2.141 | |
2001 | 3.506 | 2.112 | |
2002 | 3.587 | 2.324 | |
2003 | 3.421 | 2.223 | |
2004 | 3.841 | 2.187 | |
2005 | 4.413 | 2.205 | |
2006 | 4.662 | 2.229 | |
2007 | 5.202 | 2.134 | |
2008 | 5.06 | 2.121 | |
2009 | 4.958 | 2.199 | |
2010 | 5.167 | 2.14 | |
2011 | 16.1 | 5.478 | 2.326 |
2012 | 17.4 | 6.211 | 2.373 |
2013 | 19.3 | 5.993 | 2.446 |
2014 | 20.9 | 6.294 | 2.568 |
2015 | 22.4 | 6.775 | 2.602 |
2016 | 23.5 | 7.492 | 2.608 |
2017 | 24 | 8.127 | 2.634 |
2018 | 24.4 | 7.468 | 2.652 |
2019 | 24.8 | 6.976 | 2.682 |
2020 | 25 | ||
2021 |
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