Guoping Li;Zhigang Liu;斌 廖;南山 钟
Southwest Medical University;Shenzhen University;Guangzhou Medical College;China Association for Science and Technology
发表时间:2009-2
期 刊:Cellular and Molecular Immunology
语 言:English
U R L: http://www.scopus.com/inward/record.url?scp=65549110410&partnerID=8YFLogxK
This study was to prepare the chitosan-pDer p 2 nanoparticles and to investigate the effect of chitosan-DNA nanoparticles on immune response in mice by oral delivery of chitosan-DNA nanoparticles. The nanoparticles were synthesized by complexing chitosan with plasmid DNA. The DNA was fully complexed into chitosan-DNA nanoparticles, suggesting a 100% encapsulation efficiency. Chitosan-DNA complex renders a significant protection of the plasmid. No effect on cell viability was observed in both cell types and average cell viability over 100% was obtained. Oral gene delivery with chitosan-DNA nanoparticles can generate a higher level expression of gene in vivo. Oral chitosan-pDer p 2 nanoparticles in BALB/c mice can induce IFN-γ in serum and prevent subsequent sensitization of Th2 cell-regulated specific IgE responses. The data indicate that the oral administration of chitosan-pDer p 2 nanoparticles results in the expression of Der p 2 in the epithelial cells of both stomach and small intestine and the induction of Th1-type immune response.
医学与生命科学
Scopus度量
年份 | CiteScore | SJR | SNIP |
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1996 | |||
1997 | |||
1998 | |||
1999 | |||
2000 | |||
2001 | |||
2002 | |||
2003 | |||
2004 | |||
2005 | 0.11 | ||
2006 | 0.344 | ||
2007 | 1.057 | ||
2008 | 1.246 | ||
2009 | 1.14 | 0.335 | |
2010 | 1.265 | 0.65 | |
2011 | 4.1 | 1.333 | 0.804 |
2012 | 5.2 | 1.356 | 0.929 |
2013 | 6.4 | 1.683 | 0.912 |
2014 | 6.7 | 1.822 | 1.016 |
2015 | 6.5 | 1.945 | 0.993 |
2016 | 7.5 | 1.745 | 1.169 |
2017 | 9.4 | 2.346 | 1.451 |
2018 | 11 | 2.728 | 1.715 |
2019 | 11.8 | 2.569 | 1.535 |
2020 | 10.6 |
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