Li Runfeng;Hou Yunlong;Huang Jicheng;Pan Weiqi;Ma Qinhai;Shi Yongxia;Li Chufang;Zhao Jin;Jia Zhenhua;Jiang Haiming;Zheng Kui;Huang Shuxiang;Dai Jun;Li Xiaobo;Hou Xiaotao;Wang Lin;南山 钟;Yang Zifeng
Guangzhou Medical College;Hebei University of Chinese Medicine;Technology Centre;KingMed Virology Diagnostic & Translational Center;China Association for Science and Technology;Macau University of Science and Technology
发表时间:2020-6
期 刊:Pharmacological Research
语 言:English
U R L: http://www.scopus.com/inward/record.url?scp=85083017858&partnerID=8YFLogxK
Purpose: Lianhuaqingwen (LH) as traditional Chinese medicine (TCM) formula has been used to treat influenza and exerted broad-spectrum antiviral effects on a series of influenza viruses and immune regulatory effects Ding et al. (2017). The goal of this study is to demonstrate the antiviral activity of LH against the novel SARS-CoV-2 virus and its potential effect in regulating host immune response. Methods: The antiviral activity of LH against SARS-CoV-2 was assessed in Vero E6 cells using CPE and plaque reduction assay. The effect of LH on virion morphology was visualized under transmission electron microscope. Pro-inflammatory cytokine expression levels upon SARS-CoV-2 infection in Huh-7 cells were measured by real-time quantitative PCR assays. Results: LH significantly inhibited SARS-CoV-2 replication in Vero E6 cells and markedly reduced pro-inflammatory cytokines (TNF-α, IL-6, CCL-2/MCP-1 and CXCL-10/IP-10) production at the mRNA levels. Furthermore, LH treatment resulted in abnormal particle morphology of virion in cells. Conclusions: LH significantly inhibits the SARS-COV-2 replication, affects virus morphology and exerts anti-inflammatory activity in vitro. These findings indicate that LH protects against the virus attack, making its use a novel strategy for controlling the COVID-19 disease.
医学与生命科学
Scopus度量
年份 | CiteScore | SJR | SNIP |
---|---|---|---|
1996 | |||
1997 | |||
1998 | |||
1999 | 0.308 | 0.624 | |
2000 | 0.372 | 0.554 | |
2001 | 0.437 | 0.594 | |
2002 | 0.499 | 0.73 | |
2003 | 0.528 | 0.736 | |
2004 | 0.649 | 0.803 | |
2005 | 0.805 | 0.988 | |
2006 | 0.992 | 1.081 | |
2007 | 0.986 | 1.129 | |
2008 | 1.191 | 1.176 | |
2009 | 1.333 | 1.135 | |
2010 | 1.479 | 1.397 | |
2011 | 6.6 | 1.488 | 1.394 |
2012 | 7.8 | 1.493 | 1.637 |
2013 | 9 | 1.702 | 1.438 |
2014 | 9 | 2.031 | 1.328 |
2015 | 8.5 | 2.143 | 1.529 |
2016 | 7 | 2.037 | 1.362 |
2017 | 7.4 | 1.811 | 1.268 |
2018 | 8.9 | 1.781 | 1.433 |
2019 | 9.9 | 1.609 | 1.499 |
2020 | 9.9 | ||
2021 |
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