Objective To simplify the steps of cloning, sequencing and expressing of heterogeneous genes, a new integral plasmid pLCM182 has been designed and constructed. Methods This new plasmid is characterized with the pL promoter and RBS of phage T7 gene 10 for high expression of the inserted gene, the universal sequencing primer sites for directly sequencing the inserted gene, and a multiple cloning site specified for fitting the optimizing of translation. Thus once cloning of a PCR product into this plasmid, the gene expression and sequencing can be done without the need of subcloning, and much more time can be saved. Results By use of this plasmid, several genes obtained by PCR, including human IL-17, human IL-4, and human IL-1RA have been cloned, sequenced, and highly expressed. Conclusion The integrative plasmid is applicable and convenient for cytokines expression.