W. Zhang;雪涛 曹
Second Military Medical University
发表时间:1998-1
期 刊:Zhonghua yi xue za zhi
语 言:English
U R L: http://www.scopus.com/inward/record.url?scp=0031597881&partnerID=8YFLogxK
OBJECTIVE: To construct the recombinant adenovirus vector co-expressing the heterodimer of human interkeukin-12. METHODS: The full-length cDNA encoding human IL-12 subunits p40 or p35 was cloned by RT-PCR separately. The cDNA was ligated with encephalomyocarditis internal ribosome entry site (IRES), placed under the control of CMV promoter, and inserted into E1-substituted adenovirus vector pAx1cw to produce the bicistronic coexpression vector. Subsequently, the hIL-12 recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I-digested Ad5 DNA-TPC, and the replication-deficient hIL-12 recombinant adenoviruses was generated efficiently by homologous recombination. RESULTS: The human IL-12 recombinant adenoviruses were obtained with the titers of 2.1 x 10(9) pfu/ml. 48 hours after the infection of the 293 cells, HepG2 cells and human primary skin fibroblasts with hIL-12 recombinant adenoviruses, the hIL-12 expressions were detected by ELISA (30-50 ng/10(6) cells/24 h). The expressed hIL-12 could stimulate the in vitro proliferation and IFN-gamma production of human PMNC. CONCLUSION: Prepared hIL-12 recombinant adenovirus vector can express biologically active hIL-12 and can be potentially used in cancer gene therapy.
医学与生命科学
Scopus度量
年份 | CiteScore | SJR | SNIP |
---|---|---|---|
1996 | |||
1997 | |||
1998 | |||
1999 | 0.106 | ||
2000 | 0.11 | ||
2001 | 0.106 | ||
2002 | 0.111 | 0 | |
2003 | 0.122 | 0 | |
2004 | 0.138 | 0 | |
2005 | 0.173 | ||
2006 | 0.15 | 0.078 | |
2007 | 0.137 | 0.106 | |
2008 | 0.132 | 0.078 | |
2009 | 0.144 | 0.108 | |
2010 | 0.122 | 0.088 | |
2011 | 0.5 | 0.118 | 0.082 |
2012 | 0.2 | 0.109 | 0.087 |
2013 | 0.2 | 0.127 | 0.101 |
2014 | 0.2 | 0.138 | 0.104 |
2015 | 0.3 | 0.15 | 0.091 |
2016 | 0.3 | 0.151 | 0.098 |
2017 | 0.3 | 0.143 | 0.093 |
2018 | 0.3 | 0.153 | 0.082 |
2019 | 0.3 | 0.155 | 0.106 |
2020 | 0.4 |
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