Sanjeevi Sivasankar;Yunxiang Zhang;W. James Nelson;棣文 朱
Iowa State University;University of California at Berkeley;Stanford University;China Association for Science and Technology
发表时间:2009-8-12
期 刊:Structure
语 言:English
U R L: http://www.scopus.com/inward/record.url?scp=68149168020&partnerID=8YFLogxK
Cadherins are Ca2+-dependent cell-cell adhesion proteins with an extracellular region of five domains (EC1 to EC5). Adhesion is mediated by "strand swapping" of a conserved tryptophan residue in position 2 between EC1 domains of opposing cadherins, but the formation of this structure is not well understood. Using single-molecule fluorescence resonance energy transfer and single-molecule force measurements with the atomic force microscope, we demonstrate that cadherins initially interact via EC1 domains without swapping tryptophan-2 to form a weak Ca2+ dependent initial encounter complex that has 25% of the bond strength of a strand-swapped dimer. We suggest that cadherin dimerization proceeds via an induced fit mechanism where the monomers first form a tryptophan-2 independent initial encounter complex and then undergo subsequent conformational changes to form the final strand-swapped dimer.
医学与生命科学
Scopus度量
年份 | CiteScore | SJR | SNIP |
---|---|---|---|
1996 | |||
1997 | |||
1998 | |||
1999 | 6.698 | 2.148 | |
2000 | 5.692 | 1.936 | |
2001 | 5.194 | 1.838 | |
2002 | 4.741 | 1.841 | |
2003 | 4.797 | 1.645 | |
2004 | 4.47 | 1.506 | |
2005 | 3.746 | 1.187 | |
2006 | 4.178 | 1.297 | |
2007 | 4.424 | 1.273 | |
2008 | 4.294 | 1.355 | |
2009 | 4.847 | 1.485 | |
2010 | 4.704 | 1.477 | |
2011 | 10.8 | 4.924 | 1.577 |
2012 | 10.1 | 4.354 | 1.584 |
2013 | 10.2 | 5.137 | 1.535 |
2014 | 10.8 | 4.544 | 1.46 |
2015 | 10 | 4.659 | 1.47 |
2016 | 9 | 3.975 | 1.229 |
2017 | 8.8 | 3.554 | 1.202 |
2018 | 9.5 | 3.574 | 1.241 |
2019 | 8.6 | 2.786 | 1.202 |
2020 | 8 |
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