Alexandros Pertsinidis;Konark Mukherjee;Manu Sharma;Zhiping P. Pang;Sang Ryul Park;Yunxiang Zhang;Axel T. Brunger;Thomas C. Südhof;棣文 朱
Memorial Sloan-Kettering Cancer Center;University of California at Berkeley;Stanford University;Virginia Polytechnic Institute and State University
发表时间:2013-7-2
期 刊:Proceedings of the National Academy of Sciences of the United States of America
语 言:English
U R L: http://www.scopus.com/inward/record.url?scp=84880650864&partnerID=8YFLogxK
Membrane fusion is mediated by complexes formed by SNAPreceptor (SNARE) and Secretory 1 (Sec1)/mammalian uncoordinated- 18 (Munc18)-like (SM) proteins, but it is unclear when and how these complexes assemble. Here we describe an improved two-color fluorescence nanoscopy technique that can achieve effective resolutions of up to 7.5-nm full width at half maximum (3.2-nm localization precision), limited only by stochastic photon emission from single molecules. We use this technique to dissect the spatial relationships between the neuronal SM protein Munc18-1 and SNARE proteins syntaxin-1 and SNAP-25 (25 kDa synaptosome associated protein). Strikingly, we observed nanoscale clusters consisting of syntaxin-1 and SNAP-25 that contained associated Munc18-1. Rescue experiments with syntaxin-1 mutants revealed that Munc18-1 recruitment to the plasma membrane depends on the Munc18-1 binding to the N-terminal peptide of syntaxin-1. Our results suggest that in a primary neuron, SNARE/SM protein complexes containing syntaxin-1, SNAP-25, and Munc18-1 are preassembled in microdomains on the presynaptic plasma membrane. Our superresolution imaging method provides a framework for investigating interactions between the synaptic vesicle fusion machinery and other subcellular systems in situ.
医学与生命科学
Scopus度量
年份 | CiteScore | SJR | SNIP |
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1996 | |||
1997 | |||
1998 | |||
1999 | 8.52 | 2.398 | |
2000 | 7.927 | 2.452 | |
2001 | 7.189 | 2.458 | |
2002 | 7.281 | 2.498 | |
2003 | 7.129 | 2.507 | |
2004 | 7.197 | 2.617 | |
2005 | 6.94 | 2.546 | |
2006 | 6.849 | 2.45 | |
2007 | 6.867 | 2.46 | |
2008 | 7.034 | 2.431 | |
2009 | 7.025 | 2.552 | |
2010 | 6.898 | 2.541 | |
2011 | 16.8 | 6.864 | 2.632 |
2012 | 17.3 | 6.868 | 2.679 |
2013 | 17.4 | 7.073 | 2.721 |
2014 | 17.2 | 6.898 | 2.706 |
2015 | 17.8 | 6.814 | 2.664 |
2016 | 18 | 6.576 | 2.648 |
2017 | 17.1 | 6.092 | 2.655 |
2018 | 16.1 | 5.601 | 2.598 |
2019 | 15.7 | 5.165 | 2.689 |
2020 | 15.1 |
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