小威 庄;Taekjip Ha;Harold D. Kim;Thomas Centner;Siegfried Labeit;棣文 朱
Stanford University;University of Illinois at Urbana-Champaign;European Molecular Biology Laboratory;Washington State University Pullman
发表时间:2000-12-19
期 刊:Proceedings of the National Academy of Sciences of the United States of America
语 言:English
U R L: http://www.scopus.com/inward/record.url?scp=0034687786&partnerID=8YFLogxK
By using titin as a model system, we have demonstrated that fluorescence quenching can be used to study protein folding at the single molecule level. The unfolded titin molecules with multiple dye molecules attached are able to fold to the native state. In the native folded state, the fluorescence from dye molecules is quenched due to the close proximity between the dye molecules. Unfolding of the titin leads to a dramatic increase in the fluorescence intensity. Such a change makes the folded and unfolded states of a single titin molecule clearly distinguishable and allows us to measure the folding dynamics of individual titin molecules in real time. We have also shown that fluorescence quenching can signal folding and unfolding of a small protein with only one immunoglobulin domain.
Scopus度量
年份 | CiteScore | SJR | SNIP |
---|---|---|---|
1996 | |||
1997 | |||
1998 | |||
1999 | 8.52 | 2.398 | |
2000 | 7.927 | 2.452 | |
2001 | 7.189 | 2.458 | |
2002 | 7.281 | 2.498 | |
2003 | 7.129 | 2.507 | |
2004 | 7.197 | 2.617 | |
2005 | 6.94 | 2.546 | |
2006 | 6.849 | 2.45 | |
2007 | 6.867 | 2.46 | |
2008 | 7.034 | 2.431 | |
2009 | 7.025 | 2.563 | |
2010 | 6.898 | 2.541 | |
2011 | 16.8 | 6.864 | 2.642 |
2012 | 17.3 | 6.868 | 2.679 |
2013 | 17.4 | 7.073 | 2.731 |
2014 | 17.2 | 6.898 | 2.702 |
2015 | 17.8 | 6.814 | 2.677 |
2016 | 18 | 6.576 | 2.662 |
2017 | 17.1 | 6.092 | 2.675 |
2018 | 16.1 | 5.601 | 2.619 |
2019 | 15.7 | 5.165 | 2.695 |
2020 | 16.2 | 5.011 | 2.904 |
2021 | 13.7 |
相似文献推荐