佳杰 刁;Patricia Grob;Daniel J. Cipriano;Minjoung Kyoung;Yunxiang Zhang;Sachi Shah;Amie Nguyen;Mark Padolina;Ankita Srivastava;Marija Vrljic;Ankita Shah;Eva Nogales;棣文 朱;Axel T. Brunger
Stanford University;University of Maryland; Baltimore County;University of California at Berkeley;Lawrence Berkeley National Laboratory
发表时间:2012-12-13
期 刊:eLife
语 言:English
U R L: http://www.scopus.com/inward/record.url?scp=84881514823&partnerID=8YFLogxK
The molecular underpinnings of synaptic vesicle fusion for fast neurotransmitter release are still unclear. Here, we used a single vesicle-vesicle system with reconstituted SNARE and synaptotagmin-1 proteoliposomes to decipher the temporal sequence of membrane states upon Ca2+-injection at 250-500 μM on a 100-ms timescale. Furthermore, detailed membrane morphologies were imaged with cryo-electron microscopy before and after Ca2+-injection. We discovered a heterogeneous network of immediate and delayed fusion pathways. Remarkably, all instances of Ca2+-triggered immediate fusion started from a membrane-membrane point-contact and proceeded to complete fusion without discernible hemifusion intermediates. In contrast, pathways that involved a stable hemifusion diaphragm only resulted in fusion after many seconds, if at all. When complexin was included, the Ca2+-triggered fusion network shifted towards the immediate pathway, effectively synchronizing fusion, especially at lower Ca2+-concentration. Synaptic proteins may have evolved to select this immediate pathway out of a heterogeneous network of possible membrane fusion pathways.
Scopus度量
年份 | CiteScore | SJR | SNIP |
---|---|---|---|
1996 | |||
1997 | |||
1998 | |||
1999 | |||
2000 | |||
2001 | |||
2002 | |||
2003 | |||
2004 | |||
2005 | |||
2006 | |||
2007 | |||
2008 | |||
2009 | |||
2010 | |||
2011 | |||
2012 | 0.2 | ||
2013 | 2.2 | 6.086 | 1.533 |
2014 | 5.2 | 7.888 | 1.623 |
2015 | 6.9 | 7.741 | 1.696 |
2016 | 8.8 | 7.296 | 1.6 |
2017 | 10 | 7.121 | 1.683 |
2018 | 10.8 | 6.617 | 1.752 |
2019 | 10.8 | 6.079 | 1.686 |
2020 | 10.6 | 5.879 | 1.758 |
2021 | 9.4 |
相似文献推荐