A cytochemical method for light and electron microscopic demonstration of ferric reductase

He Wang;Cui Lin Yi;福锁 张

China Agricultural University

发表时间:1997

期 刊:Acta Botanica Sinica

语 言:English

U R L: http://www.scopus.com/inward/record.url?scp=5244269126&partnerID=8YFLogxK

摘要

The soybean ( Glycine max L. ) root cultured under iron deficient condition was employed as the experimental material in order to raise the ferric reductase content in root. The method is chemically based on the reduction of ferricyanide by ferric reductase to ferrocyanide from which, in presense of cupric ions, an insoluble and electron-dense brownish precipitate is formed. For better observation under light microscope, the dull brown precipitate was converted into black silver precipitate of sharp-contrast using the sulfide-silver amplification method. With this method, accurrate and specific localization of ferric reductase was obtained. Under electron microscope, the dense enzymic reaction product was found as fine granules covering the plasmalemma which was in consistent with the results obtained by biochemical method. The present method for the localization of ferric reductase is more accurrate than the prussian blue staining method, because the brown precipitate in this method is finer than the prussian blue precipitate. Further, the pH 6.6 of the reaction medium is closer to the optimum pH of 5.5 to 6.5 of ferric reductase activity whereas the pH of the prussian blue staining solution is only 3.

关键词

Cytochemistry
Ferric reductase
Ferricyanide
Iron deficency
Soybean

相关科学

农业与生物科学
植物科学
生物化学、遗传学和分子生物学
生物化学

文献指纹

化合物

ferric citrate iron reductase

Precipitates

ferric ferrocyanide

Demonstrations

Electrons

silver sulfide

hexacyanoferrate II

Reaction products

Silver

Amplification

Glycine

Microscopes

Electron microscopes

Iron

Ions

医学与生命科学

ferric citrate iron reductase

Electrons

Light

ferric ferrocyanide

Soybeans

silver sulfide

ferricyanide reductase

hexacyanoferrate II

Staining and Labeling

Silver

Iron

Ions

Observation

农业与生物学

electrons

silver

methodology

staining

ferrocyanides

ferricyanides

enzymatic reactions

light microscopes

electron microscopes

sulfides

Glycine max

granules

plasma membrane

iron

soybeans

ions

期刊度量

Scopus度量

年份 CiteScore SJR SNIP
1996
1997
1998
1999 0.213 0.485
2000 0.236 0.369
2001 0.182 0.263
2002 0.195 0.484
2003 0.181 0.638
2004 0.207 0.452
2005 0.247 0.383
2006 0.286 0.494
2007 0.297 0.451
2008 0.371 0.53
2009 0.669 0.721
2010 0.689 0.793
2011 4.2 1.132 0.94
2012 5.3 1.308 1.031
2013 7.2 1.731 1.184
2014 6.1 1.599 1.093
2015 6.6 1.717 1.059
2016 7.5 1.841 1.12
2017 8.1 1.782 1.135
2018 7.3 1.848 1.185
2019 8.1 2.32 1.619
2020 8.4

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