J. C. Reddy;J. C. Morris;J. Wang;M. A. English;D. A. Haber;一公 施;J. D. Licht
Icahn School of Medicine at Mount Sinai;China Association for Science and Technology
发表时间:1995
期 刊:Journal of Biological Chemistry
语 言:English
U R L: http://www.scopus.com/inward/record.url?scp=0028903902&partnerID=8YFLogxK
The WT1 tumor suppressor gene encodes four isoforms of a zinc finger transcription factor with both activation and repression functions which are dependent upon promoter architecture. Using a simple HSV-tk promoter containing 5'-Egr-1/WT1-binding sites, we found that WT1 isoforms (A) and (B) strongly activated transcription. WT1(A) and (B) bound equally well to the Egr-1/WT1-binding site, but WT1(B), which contains a 17 amino acid insertion compared to WT1(A), was a consistently stronger activator of transcription than WT1(A). Transcriptional activation by wild-type WT1 was inhibited by coexpression of WT(PM) or WT(AR), genetically defined dominant negative alleles of WT1. In vitro, as well as in the yeast two-hybrid system, WT1 protein associated with itself and with dominant negative mutant proteins. The major domain required for self-association and inhibition of transcriptional activation mapped to the first 182 amino acids of WT1. Dominant negative WT1 alleles may play a role in tumorigenesis by associating with wild-type WT1 proteins and decreasing their transcriptional activity.
化合物
医学与生命科学
Scopus度量
年份 | CiteScore | SJR | SNIP |
---|---|---|---|
1996 | |||
1997 | |||
1998 | |||
1999 | 6.161 | 1.611 | |
2000 | 5.435 | 1.559 | |
2001 | 4.869 | 1.505 | |
2002 | 4.494 | 1.42 | |
2003 | 4.512 | 1.416 | |
2004 | 4.376 | 1.42 | |
2005 | 4.178 | 1.351 | |
2006 | 4.352 | 1.379 | |
2007 | 4.338 | 1.379 | |
2008 | 4.375 | 1.362 | |
2009 | 4.235 | 1.348 | |
2010 | 3.998 | 1.341 | |
2011 | 8.3 | 3.544 | 1.253 |
2012 | 8.3 | 3.396 | 1.235 |
2013 | 8.9 | 3.402 | 1.218 |
2014 | 8.8 | 3.258 | 1.206 |
2015 | 8.8 | 3.126 | 1.18 |
2016 | 8.5 | 2.825 | 1.129 |
2017 | 8.2 | 2.672 | 1.096 |
2018 | 7.7 | 2.403 | 1.089 |
2019 | 7.4 | 2.283 | 1.089 |
2020 | 7.6 | ||
2021 |
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