Mengying Zhou;Yini Li;Qi Hu;Xiao Chen Bai;Weiyun Huang;Chuangye Yan;Sjors H.W. Scheres;一公 施
Tsinghua University;Medical Research Council
发表时间:2015-11-15
期 刊:Genes and Development
语 言:English
U R L: http://www.scopus.com/inward/record.url?scp=84947921566&partnerID=8YFLogxK
The apoptotic protease-activating factor 1 (Apaf-1) controls the onset of many known forms of intrinsic apoptosis in mammals. Apaf-1 exists in normal cells as an autoinhibited monomer. Upon binding to cytochrome c and dATP, Apaf-1 oligomerizes into a heptameric complex known as the apoptosome, which recruits and activates cell-killing caspases. Here we present an atomic structure of an intact mammalian apoptosome at 3.8 Å resolution, determined by single-particle, cryo-electron microscopy (cryo-EM). Structural analysis, together with structure-guided biochemical characterization, uncovered how cytochrome c releases the autoinhibition of Apaf-1 through specific interactions with the WD40 repeats. Structural comparison with autoinhibited Apaf-1 revealed how dATP binding triggers a set of conformational changes that results in the formation of the apoptosome. Together, these results constitute the molecular mechanism of cytochrome c- and dATP-mediated activation of Apaf-1.
医学与生命科学
Scopus度量
年份 | CiteScore | SJR | SNIP |
---|---|---|---|
1996 | |||
1997 | |||
1998 | |||
1999 | 25.272 | 3.088 | |
2000 | 22.2 | 3.036 | |
2001 | 20.005 | 3.12 | |
2002 | 20.117 | 3.099 | |
2003 | 18.492 | 2.923 | |
2004 | 16.983 | 2.833 | |
2005 | 15.979 | 2.576 | |
2006 | 15.479 | 2.597 | |
2007 | 17.565 | 2.691 | |
2008 | 15.64 | 2.352 | |
2009 | 15.51 | 2.429 | |
2010 | 14.948 | 2.389 | |
2011 | 23.2 | 13.725 | 2.314 |
2012 | 22.5 | 12.943 | 2.417 |
2013 | 23 | 14.209 | 2.507 |
2014 | 22 | 12.005 | 2.405 |
2015 | 20.1 | 11.598 | 2.273 |
2016 | 18.5 | 10.399 | 2.055 |
2017 | 17.3 | 10.214 | 1.994 |
2018 | 16.3 | 7.925 | 1.843 |
2019 | 17 | 7.92 | 1.964 |
2020 | 15.8 | ||
2021 |
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