Nieng Yan;立川 谷;David Kokel;继杰 柴;Wenyu Li;爱东 韩;Lin Chen;定 薛;一公 施
Princeton University;China Association for Science and Technology;University of Colorado Boulder
发表时间:2004-9-24
期 刊:Molecular Cell
语 言:English
U R L: http://www.scopus.com/inward/record.url?scp=4644249309&partnerID=8YFLogxK
Programmed cell death in Caenorhabditis elegans is initiated by the binding of EGL-1 to CED-9, which disrupts the CED-4/CED-9 complex and allows CED-4 to activate the cell-killing caspase CED-3. Here we demonstrate that the C-terminal half of EGL-1 is necessary and sufficient for binding to CED-9 and for killing cells. Structure of the EGL-1/CED-9 complex revealed that EGL-1 adopts an extended α-helical conformation and induces substantial structural rearrangements in CED-9 upon binding. EGL-1 interface mutants failed to bind to CED-9 or to release CED-4 from the CED-4/CED-9 complex, and were unable to induce cell death in vivo. A surface patch on CED-9, different from that required for binding to EGL-1, was identified to be responsible for binding to CED-4. These data suggest a working mechanism for the release of CED-4 from the CED-4/CED-9 complex upon EGL-1 binding and provide a mechanistic framework for understanding apoptosis activation in C. elegans.
Scopus度量
年份 | CiteScore | SJR | SNIP |
---|---|---|---|
1996 | |||
1997 | |||
1998 | |||
1999 | 20.282 | 2.834 | |
2000 | 18.871 | 2.754 | |
2001 | 16.477 | 2.752 | |
2002 | 17.683 | 2.645 | |
2003 | 16.202 | 2.529 | |
2004 | 16.775 | 2.638 | |
2005 | 15.371 | 2.411 | |
2006 | 14.444 | 2.497 | |
2007 | 14.755 | 2.457 | |
2008 | 14.891 | 2.539 | |
2009 | 17.237 | 2.818 | |
2010 | 15.376 | 2.787 | |
2011 | 24.6 | 14.472 | 2.828 |
2012 | 25.7 | 13.492 | 2.853 |
2013 | 27.5 | 14.689 | 3.056 |
2014 | 24.8 | 12.999 | 2.735 |
2015 | 24.5 | 13.587 | 2.778 |
2016 | 25.8 | 13.619 | 2.759 |
2017 | 26.1 | 13.841 | 2.849 |
2018 | 24.9 | 12.076 | 2.858 |
2019 | 24.2 | 11.075 | 2.916 |
2020 | 25.5 | ||
2021 |
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