Wenyu Li;Srinivasa M. Srinivasula;继杰 柴;Pingwei Li;Jia Wei Wu;Zhijia Zhang;Emad S. Alnemri;一公 施
Princeton University;Thomas Jefferson University;China Association for Science and Technology
发表时间:2002
期 刊:Nature Structural Biology
语 言:English
U R L: http://www.scopus.com/inward/record.url?scp=0036263973&partnerID=8YFLogxK
HtrA2/Omi, a mitochondrial serine protease in mammals, is important in programmed cell death. However, the underlining mechanism of HtrA2/Omi-mediated apoptosis remains unclear. Analogous to the bacterial homolog HtrA (DegP), the mature HtrA2 protein contains a central serine protease domain and a C-terminal PDZ domain. The 2.0 Å crystal structure of HtrA2/Omi reveals the formation of a pyramid-shaped homotrimer mediated exclusively by the serine protease domains. The peptide-binding pocket of the PDZ domain is buried in the intimate interface between the PDZ and the protease domains. Mutational analysis reveals that the monomeric HtrA2/Omi mutants are unable to induce cell death and are deficient in protease activity. The PDZ domain modulates HtrA2/Omi-mediated cell death activity by regulating its serine protease activity. These structural and biochemical observations provide an important framework for deciphering the mechanisms of HtrA2/Omi-mediated apoptosis.
Scopus度量
年份 | CiteScore | SJR | SNIP |
---|---|---|---|
1996 | |||
1997 | |||
1998 | |||
1999 | 8.539 | 2.761 | |
2000 | 6.981 | 2.556 | |
2001 | 6.543 | 2.365 | |
2002 | 6.832 | 2.636 | |
2003 | 6.992 | 2.568 | |
2004 | 7.262 | 2.696 | |
2005 | 8.122 | 2.391 | |
2006 | 8.369 | 2.297 | |
2007 | 9.705 | 2.213 | |
2008 | 9.602 | 2.034 | |
2009 | 12.133 | 2.351 | |
2010 | 12.732 | 2.608 | |
2011 | 20.4 | 11.824 | 2.517 |
2012 | 22 | 12.177 | 2.607 |
2013 | 21.4 | 11.168 | 2.452 |
2014 | 21.1 | 10.155 | 2.359 |
2015 | 23.2 | 11.288 | 2.466 |
2016 | 23.3 | 11.42 | 2.512 |
2017 | 20.9 | 10.873 | 2.539 |
2018 | 20.5 | 10.005 | 2.522 |
2019 | 19.1 | 8.792 | 2.254 |
2020 | 19.3 | 9.448 | 2.536 |
2021 | 19.7 |
相似文献推荐