Hongxu Qin;Srinivasa M. Srinivasula;更 吴;Teresa Fernandes-Alnemri;Emad S. Alnemri;一公 施
Princeton University;Thomas Jefferson University
发表时间:1999-6-10
期 刊:Nature
语 言:English
U R L: http://www.scopus.com/inward/record.url?scp=0033542482&partnerID=8YFLogxK
Caspase-9-mediated apoptosis (programmed cell death) plays a central role in the development and homeostasis of all multicellular organisms. Mature caspase-9 is derived from its procaspase precursor as a result of recruitment by the activating factor Apaf-1. The crystal structures of the caspase-recruitment domain of Apaf-1 by itself and in complex with the prodomain of procaspase-9 have been determined at 1.6 and 2.5 A resolution, respectively. These structures and other evidence reveal that each molecule of Apaf-1 interacts with a molecule of procaspase-9 through two highly charged and complementary surfaces formed by non-conserved residues; these surfaces determine recognition specificity through networks of intermolecular hydrogen bonds and van der Waals interactions. Mutation of the important interface residues in procaspase-9 or Apaf-1 prevents or reduces activation of procaspase-9 in a cell-free system. Wild-type, but not mutant, prodomains of caspase-9 completely inhibit catalytic processing of procaspase-9. Furthermore, analysis of homologues from Caenorhabditis elegans indicates that recruitment of CED-3 by CED-4 is probably mediated by the same set of conserved structural motifs, with a corresponding change in the specificity- determining residues.
Scopus度量
年份 | CiteScore | SJR | SNIP |
---|---|---|---|
1996 | |||
1997 | |||
1998 | |||
1999 | 15.599 | 7.187 | |
2000 | 11.917 | 6.857 | |
2001 | 9.874 | 6.767 | |
2002 | 10.114 | 7.184 | |
2003 | 11.384 | 7.492 | |
2004 | 11.222 | 7.538 | |
2005 | 10.333 | 7.199 | |
2006 | 9.702 | 7.156 | |
2007 | 10.344 | 7.097 | |
2008 | 13.17 | 7.307 | |
2009 | 15.185 | 8.234 | |
2010 | 16.465 | 8.204 | |
2011 | 53.1 | 17.598 | 8.667 |
2012 | 51 | 17.546 | 8.409 |
2013 | 50.9 | 19.69 | 8.511 |
2014 | 49.9 | 18.78 | 7.918 |
2015 | 51.6 | 19.669 | 8.08 |
2016 | 49.2 | 18.389 | 7.901 |
2017 | 53.7 | 17.875 | 8.679 |
2018 | 55.7 | 16.345 | 9.448 |
2019 | 51 | 14.047 | 8.546 |
2020 | 56.9 | 15.993 | 9.249 |
2021 | 56 |
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